Gaining insight into plant gene transcription using smFISH

Transcription. 2018;9(3):166-170. doi: 10.1080/21541264.2017.1372043. Epub 2017 Nov 3.

Abstract

Single molecule RNA fluorescent in situ hybridization (smFISH) enables gene transcription to be assessed at the cellular level. In this point of view article, we describe our recent smFISH research in the model plant Arabidopsis thaliana and discuss how this technique could further knowledge of plant gene transcription in the future.

Keywords: Arabidopsis; RNA Imaging; lncRNA; mRNA Quantification; smFISH.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / genetics*
  • In Situ Hybridization, Fluorescence / methods*
  • RNA, Long Noncoding / genetics
  • RNA, Messenger / genetics
  • RNA, Plant / genetics*
  • Transcription, Genetic*

Substances

  • RNA, Long Noncoding
  • RNA, Messenger
  • RNA, Plant