Biomarkers of oxidative stress and protein-protein interaction in chronic obstructive pulmonary disease

Arch Physiol Biochem. 2018 Jul;124(3):226-231. doi: 10.1080/13813455.2017.1387796. Epub 2017 Oct 11.

Abstract

Content: The increased oxidative stress in chronic obstructive pulmonary disease (COPD) patients is the result of increased inhaled oxidants, generated by various cells of the airways.

Objective: The investigation included measurements of malondiadehyde (MDA), uric acid, ascorbic acid, and matrix metalloproteinase-12 (MMP-12) in COPD patient. We also performed genetic analysis for protein-protein interaction (PPI) network.

Materials and methods: The study was conducted on healthy subjects with normal lung function (NS, 14 subjects) and 28 patients (Global Initiative for Chronic Obstructive Lung Disease (Gold) 1 and Gold 2) with COPD.

Results: There was significant (p < .001) increase in MMP-12, MDA and uric acid levels as compared to healthy controls. A significant (p < .001) decline in ascorbic acid level was observed in COPD patients. The PPI was found to be 0.833 which indicated that proteins present in COPD are linked.

Discussion and conclusion: This study suggests oxidative stress plays an important role in COPD and the PPI provide indication that proteins present in COPD are linked.

Keywords: Chronic obstructive pulmonary disease; MMP-12; MDA; PPI network; ascorbic acid; oxidative stress; uric acid.

MeSH terms

  • Ascorbic Acid / blood
  • Case-Control Studies
  • Female
  • Gene Expression Regulation, Enzymologic
  • Humans
  • Male
  • Malondialdehyde / blood
  • Matrix Metalloproteinase 12 / blood
  • Middle Aged
  • Oxidative Stress*
  • Protein Interaction Maps*
  • Pulmonary Disease, Chronic Obstructive / blood
  • Pulmonary Disease, Chronic Obstructive / metabolism*
  • Uric Acid / blood

Substances

  • Uric Acid
  • Malondialdehyde
  • Matrix Metalloproteinase 12
  • Ascorbic Acid