Genetic Polymorphisms of Cytochrome P450 Enzymes and Transport Proteins in a Russian Population and Three Ethnic Groups of Dagestan

Genet Test Mol Biomarkers. 2017 Dec;21(12):747-753. doi: 10.1089/gtmb.2017.0036. Epub 2017 Oct 12.

Abstract

Aim: The objective of this study was to investigate the prevalence of polymorphic markers of the CYP2C19, CYP2C9, CYP2D6, SLCO1B1, and ABCB1 genes among the three ethnic groups in Dagestan and compare it with the carrier frequency of these markers among the Russian population living in Moscow.

Methods: The study involved 186 healthy, unrelated, and chronic medication-free volunteers (53 males and 133 females) of the three ethnic groups in the Dagestan Republic: 46 Laks, 90 Avars, and 50 Dargins. Genotyping was performed using real-time polymerase chain reaction-based methods. The allelic prevalences of the three Dagestan peoples were compared with ethnic Russians from the Moscow region.

Results: Statistically significant differences for the following gene polymorphisms: CYP2C19*17, CYP2C9*3, ABCB1 (C3435T), SLCO1B1*5 were found between the Russian population and the three ethnic groups of the Dagestan republic.

Conclusion: The data obtained from this study will help with prioritization genotyping in the region.

Keywords: Avars; Dargins; Laks; P-glycoprotein; Russians; ethnic groups; pharmacogenetics; the P450 cytochrome.

MeSH terms

  • ATP Binding Cassette Transporter, Subfamily B / genetics
  • Adult
  • Alleles
  • Carrier Proteins / genetics
  • Cytochrome P-450 CYP2C19 / genetics
  • Cytochrome P-450 CYP2C9 / genetics
  • Cytochrome P-450 CYP2D6 / genetics
  • Cytochrome P-450 Enzyme System / genetics*
  • Cytochrome P-450 Enzyme System / metabolism
  • Dagestan
  • Ethnicity / genetics*
  • Female
  • Gene Frequency
  • Genotype
  • Healthy Volunteers
  • Humans
  • Liver-Specific Organic Anion Transporter 1 / genetics
  • Male
  • Polymorphism, Genetic / genetics
  • Real-Time Polymerase Chain Reaction
  • Russia
  • White People / genetics

Substances

  • ABCB1 protein, human
  • ATP Binding Cassette Transporter, Subfamily B
  • Carrier Proteins
  • Liver-Specific Organic Anion Transporter 1
  • SLCO1B1 protein, human
  • Cytochrome P-450 Enzyme System
  • CYP2C9 protein, human
  • Cytochrome P-450 CYP2C9
  • Cytochrome P-450 CYP2C19
  • Cytochrome P-450 CYP2D6