Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers

Teodora Ribarska; Gregor D Gilfillan

doi:10.1007/978-1-4939-7380-4_14

Native Chromatin Immunoprecipitation-Sequencing (ChIP-Seq) from Low Cell Numbers

Methods Mol Biol. 2018:1689:157-166. doi: 10.1007/978-1-4939-7380-4_14.

Authors

Teodora Ribarska¹, Gregor D Gilfillan²

Affiliations

¹ Molecular Biology of the Cell II, DKFZ, Im Neuenheimer Feld 280, 69221, Heidelberg, Germany.
² Department of Medical Genetics, Oslo University Hospital and University of Oslo, Kirkeveien 166, 0407, Oslo, Norway. [email protected].

PMID: 29027173
DOI: 10.1007/978-1-4939-7380-4_14

Abstract

ChIP-seq is the current method of choice for genome-wide protein location analysis. Here, we present a native (non-cross-linked) ChIP procedure suitable for histone proteins, coupled with an efficient library preparation technique for subsequent next-generation sequencing. The method enables ChIP-seq starting with 50,000 or more cells.

Keywords: HTS; Micro-ChIP; N-ChIP; NGS; Next-generation sequencing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatin Immunoprecipitation* / methods
DNA / genetics
DNA / metabolism
DNA Cleavage
Gene Library
HeLa Cells
High-Throughput Nucleotide Sequencing* / methods
Humans
Sequence Analysis, DNA

Substances

DNA