Abstract
The implementation of PCR for the detection of carbapenemase genes enables rapid results with significant epidemiological implications. Two commercial real-time PCR assays, the Hylabs Hy-CRE and Sacace MDR MBL+KPC/OXA, were evaluated for the detection of the genes blaKPC, blaVIM, blaNDM, blaIMP and blaOXA-48-carbapenemasein a collection of 96 carbapenem-resistant Enterobacteriaceae strains with different resistant mechanisms. Both assays exhibited excellent diagnostic performance, with 100 % sensitivity and specificity.
Keywords:
carbapenem-resistant Enterobacteriaceae; carbapenemase; infection control; molecular diagnostics.
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism*
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Carbapenems / pharmacology
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DNA, Bacterial
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Drug Resistance, Bacterial
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Enterobacteriaceae / enzymology*
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Enterobacteriaceae / genetics
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Enterobacteriaceae / metabolism
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Gene Expression Regulation, Bacterial / physiology*
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Gene Expression Regulation, Enzymologic / physiology*
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Real-Time Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Species Specificity
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beta-Lactamases / genetics
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beta-Lactamases / metabolism*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carbapenems
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DNA, Bacterial
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beta-Lactamases
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carbapenemase