CD80 Regulates Th17 Cell Differentiation in Coxsackie Virus B3-Induced Acute Myocarditis

Inflammation. 2018 Feb;41(1):232-239. doi: 10.1007/s10753-017-0681-7.

Abstract

The cluster of differentiation protein complex, CD80/CD86, regulates Th1/Th2 differentiation in autoimmune disease. In order to establish the effects of CD80/CD86 on Th17 cell differentiation in acute viral myocarditis (VMC), we infected C57BL/6 mice with Coxsackie virus B3 (CVB3) and examined the effects of the treatment with anti-CD80/CD86 monoclonal antibodies (mAbs) on Th17 cell differentiation in vivo. The effects of anti-CD80/CD86 mAbs on Th17 cell differentiation were further evaluated in vitro. The treatment with anti-CD80 mAb induced marked suppression of Th17 cell differentiation and ROR-γt mRNA expression, whereas anti-CD86 mAb alone had no effect, both in vivo and in vitro. Our finding that CD80 regulates Th17 differentiation supports the potential utility of anti-CD80 mAb as an effective new immunotherapeutic target in acute VMC.

Keywords: CD80; CD86; Th17 cells; differentiation; viral myocarditis.

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology
  • B7-1 Antigen / antagonists & inhibitors
  • B7-1 Antigen / immunology*
  • B7-1 Antigen / metabolism
  • B7-2 Antigen / immunology
  • B7-2 Antigen / metabolism
  • Cell Differentiation* / drug effects
  • Cells, Cultured
  • Coxsackievirus Infections / drug therapy
  • Coxsackievirus Infections / immunology*
  • Coxsackievirus Infections / metabolism
  • Coxsackievirus Infections / virology
  • Disease Models, Animal
  • Enterovirus B, Human / immunology*
  • Enterovirus B, Human / pathogenicity
  • Host-Pathogen Interactions
  • Male
  • Mice, Inbred C57BL
  • Myocarditis / drug therapy
  • Myocarditis / immunology*
  • Myocarditis / metabolism
  • Myocarditis / virology
  • Myocardium / immunology*
  • Myocardium / metabolism
  • Myocardium / pathology
  • Nuclear Receptor Subfamily 1, Group F, Member 3 / genetics
  • Nuclear Receptor Subfamily 1, Group F, Member 3 / immunology
  • Signal Transduction
  • Spleen / drug effects
  • Spleen / immunology*
  • Spleen / metabolism
  • Spleen / virology
  • Th17 Cells / drug effects
  • Th17 Cells / immunology*
  • Th17 Cells / metabolism
  • Th17 Cells / virology

Substances

  • Antibodies, Monoclonal
  • B7-1 Antigen
  • B7-2 Antigen
  • Cd86 protein, mouse
  • Nuclear Receptor Subfamily 1, Group F, Member 3
  • Rorc protein, mouse