Abstract
Transglutaminase (TGase) activity was reduced in intact mitogen-stimulated human peripheral blood lymphocytes (PBL) when compared to intact resting PBL. Moreover, a treatment of the same quiescent immunocompetent cells with purified liver TGase and Ca2+ completely suppressed the mitogen-induced blast transformation. A decrease in TGase activity in neoplastically transformed seminal vesicle epithelial cells with respect to their normal parent counterpart was also observed. Our data support the notion of a possible implication of TGase in cell proliferation and transformation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Binding, Competitive
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Calcium / pharmacology
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Catalysis
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Cell Division
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Cell Line
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Cell Transformation, Neoplastic / metabolism*
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Concanavalin A / pharmacology
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Culture Media
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Harvey murine sarcoma virus / pathogenicity
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Kirsten murine sarcoma virus / pathogenicity
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Liver / enzymology
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Lymphocytes / pathology
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Mitogens / pharmacology
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Proteins / analysis
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Rats
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Rats, Inbred Strains
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Transglutaminases / antagonists & inhibitors
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Transglutaminases / metabolism*
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Transglutaminases / physiology
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Tumor Cells, Cultured / enzymology
Substances
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Culture Media
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Mitogens
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Proteins
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Concanavalin A
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Transglutaminases
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Calcium