Determination of the absolute oral bioavailability of niraparib by simultaneous administration of a 14C-microtracer and therapeutic dose in cancer patients

Cancer Chemother Pharmacol. 2018 Jan;81(1):39-46. doi: 10.1007/s00280-017-3455-x. Epub 2017 Oct 17.

Abstract

Introduction: Niraparib (Zejula™) is a poly(ADP-ribose) polymerase inhibitor recently approved by the US Food and Drug Administration for the maintenance treatment of patients with recurrent platinum-sensitive epithelial ovarian, fallopian tube, or primary peritoneal cancer who are in a complete or partial response to platinum-based chemotherapy. The pivotal phase III clinical trial has shown improved progression-free survival in patients receiving niraparib compared with those receiving placebo.

Purpose: Since niraparib is administered orally, it is of interest to investigate the oral bioavailability (F po) of this novel compound, which is the aim of this study.

Methods: Six patients received an oral therapeutic dose of 300 mg niraparib, followed by a 15-min intravenous infusion of 100 µg 14C-niraparib with a radioactivity of approximately 100 nCi. The niraparib therapeutic dose was measured in plasma using a validated liquid chromatography-tandem mass spectrometry method, whereas the total 14C-radioactivity and 14C-niraparib plasma levels were measured by accelerator mass spectrometry and a validated high performance liquid chromatography assay with AMS.

Results: The F po of niraparib was determined to be 72.7% in humans.

Keywords: 14C-microtracer; AMS; Bioavailability; LC–MS/MS; Niraparib; Pharmacokinetics.

Publication types

  • Clinical Trial, Phase III
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Administration, Oral
  • Adult
  • Aged
  • Antineoplastic Agents / administration & dosage*
  • Antineoplastic Agents / blood
  • Antineoplastic Agents / pharmacokinetics*
  • Antineoplastic Agents / therapeutic use
  • Area Under Curve
  • Biological Availability
  • Breast Neoplasms / drug therapy*
  • Breast Neoplasms / metabolism
  • Carbon Radioisotopes / metabolism*
  • Chromatography, High Pressure Liquid
  • Fallopian Tube Neoplasms / drug therapy*
  • Fallopian Tube Neoplasms / metabolism
  • Female
  • Humans
  • Indazoles / administration & dosage*
  • Indazoles / blood
  • Indazoles / pharmacokinetics*
  • Indazoles / therapeutic use
  • Infusions, Intravenous
  • Middle Aged
  • Ovarian Neoplasms / drug therapy*
  • Ovarian Neoplasms / metabolism
  • Piperidines / administration & dosage*
  • Piperidines / blood
  • Piperidines / pharmacokinetics*
  • Piperidines / therapeutic use
  • Poly(ADP-ribose) Polymerase Inhibitors / administration & dosage*
  • Poly(ADP-ribose) Polymerase Inhibitors / pharmacokinetics*
  • Poly(ADP-ribose) Polymerase Inhibitors / therapeutic use
  • Progression-Free Survival
  • Radioactive Tracers
  • Tandem Mass Spectrometry

Substances

  • Antineoplastic Agents
  • Carbon Radioisotopes
  • Indazoles
  • Piperidines
  • Poly(ADP-ribose) Polymerase Inhibitors
  • Radioactive Tracers
  • niraparib