A simple validated multi-analyte method for detecting drugs in oral fluid by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)

Drug Test Anal. 2018 Jun;10(6):1001-1008. doi: 10.1002/dta.2325. Epub 2017 Dec 18.

Abstract

A UPLC-MS/MS method was developed to identify and quantitate 37 commonly abused drugs in oral fluid. Drugs of interest included amphetamines, benzodiazepines, cocaine, opiates, opioids, phencyclidine and tetrahydrocannabinol. Sample preparation and extraction are simple, and analysis times short. Validation showed satisfactory performance at relevant concentrations. The possibility of contaminated samples as well as the interpretation in relation to well-knows matrices, such as urine, will demand further study.

Keywords: amphetamines; benzodiazepines; opiates; ultra-performance liquid chromatography-tandem mass spectrometry; Δ9- tetrahydrocannabinol.

Publication types

  • Validation Study

MeSH terms

  • Chromatography, High Pressure Liquid
  • Humans
  • Illicit Drugs / analysis*
  • Saliva / chemistry*
  • Substance Abuse Detection / methods*
  • Tandem Mass Spectrometry

Substances

  • Illicit Drugs