Application of hydrolysis probe analysis to identify clade types of the malaria vector mosquito Anopheles funestus sensu stricto from Muheza, northeastern Tanzania

E J Kweka; E A Mausa; N Venter; Y A Derua; E E Kimaro; M Coetzee

doi:10.1111/mve.12274

Application of hydrolysis probe analysis to identify clade types of the malaria vector mosquito Anopheles funestus sensu stricto from Muheza, northeastern Tanzania

Med Vet Entomol. 2018 Mar;32(1):125-128. doi: 10.1111/mve.12274. Epub 2017 Oct 25.

Authors

E J Kweka^{1

2}, E A Mausa³, N Venter^{4

5}, Y A Derua⁶, E E Kimaro¹, M Coetzee^{4

5}

Affiliations

¹ Division of Livestock and Human Diseases Vector Control, Tropical Pesticides Research Institute, Arusha, Tanzania.
² Department of Medical Parasitology and Entomology, Catholic University of Health and Allied Sciences, Mwanza, Tanzania.
³ National Plant Genetic Resource Centre, Tropical Pesticides Research Institute, Arusha, Tanzania.
⁴ Faculty of Health Sciences, Wits Research Institute for Malaria, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa.
⁵ Centre for Emerging, Zoonotic & Parasitic Diseases, National Institute for Communicable Diseases, Johannesburg, South Africa.
⁶ National Institute for Medical Research, Amani Research Centre, Tanga, Tanzania.

PMID: 29068089
DOI: 10.1111/mve.12274

Abstract

A hydrolysis probe analysis (TaqMan assay) was used to study clade types in Anopheles funestus sensu stricto Giles, a major malaria vector in sub-Saharan Africa, with specimens collected from Muheza in Tanga, northeastern Tanzania. A total of 186 An. funestus specimens were analysed, revealing that 176 (94.6%) were of clade I and 10 (5.4%) of clade II. These findings extend the distribution of clade type II from southern Mozambique and northern Zambia to northeastern Tanzania. The technique used can also be of great value in assessing the role and contribution of these clade types in malaria transmission and insecticide resistance frequencies for An. funestus s.s.

Keywords: Anopheles funestus; Muheza; Tanzania; clade types; hydrolysis probe.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animal Distribution
Animals
Anopheles / classification*
Anopheles / genetics
Hydrolysis
Malaria / transmission
Mosquito Vectors / classification*
Mosquito Vectors / genetics
Polymerase Chain Reaction
Tanzania