Identification and characterization of a human cell line with dendritic cell features

Virchows Arch B Cell Pathol Incl Mol Pathol. 1988;56(2):77-83. doi: 10.1007/BF02890005.

Abstract

CORP-4 is a cell line obtained in our laboratory from an explanted human bladder carcinoma. This cell line shows certain dendritic cell features such as adherence to the culture plate surface, a doubling time of 24 h and an enzymatic profile typical of cells involved in antigen presentation (non-specific esterases, lysozyme and alpha-1-antitrypsin). Its phenotypic analysis revealed CD 15 and Fc receptor expression, S-100 surface protein and the presence of positive reactivity to different lectins such as Concanavalin A (Con A) and Peanut agglutinin (PNA). CORP-4 was found to be a non-phagocytic cell line after it was assayed with latex, and FcR- and C3bR-mediated phagocytosis. Furthermore, CORP-4 produced interleukin-1 (IL-1) as determined by thymocyte proliferation assays and also fixes immune complexes in a non-complement dependent fashion. HLA class I and class II antigens were inducible by both 5 azacytidine and gamma interferon.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agglutination Tests
  • Animals
  • Cell Line
  • Dendritic Cells / analysis
  • Dendritic Cells / cytology*
  • Dendritic Cells / immunology
  • Histocompatibility Antigens / analysis
  • Humans
  • Immunohistochemistry
  • In Vitro Techniques
  • Interleukin-1 / biosynthesis
  • Mice
  • Mice, Inbred C3H
  • Phagocytosis
  • Tumor Cells, Cultured
  • Urinary Bladder Neoplasms / pathology

Substances

  • Histocompatibility Antigens
  • Interleukin-1