High genetic diversity of porcine enterovirus G in Schleswig-Holstein, Germany

Arch Virol. 2018 Feb;163(2):489-493. doi: 10.1007/s00705-017-3612-x. Epub 2017 Oct 28.

Abstract

Between 2012 and 2015, 495 pooled snout swabs from fattening pigs raised in Schleswig-Holstein, Germany, were screened for the presence of enterovirus G (EV-G) RNA. Nucleic acids were tested in diverse reverse transcription polymerase chain reaction assays applying published oligonucleotide primers specific for the viral protein (VP) 1 and 2/4 encoding regions as well as for 3D polymerase. Phylogenetic analyses of VP1 revealed the presence of 12 EV-G types, three of which had highly divergent sequences suggesting putative new types. Co-circulation of EV-G types was observed in several pigsties. Thus, genetic diversity of EV-G was demonstrated in this small geographic area.

MeSH terms

  • Animals
  • Capsid Proteins / genetics
  • DNA Primers / genetics
  • Enterovirus Infections / veterinary*
  • Enterovirus Infections / virology
  • Enteroviruses, Porcine / classification
  • Enteroviruses, Porcine / genetics*
  • Enteroviruses, Porcine / isolation & purification
  • Feces / virology
  • Genetic Variation*
  • Germany
  • Phylogeny
  • Reverse Transcriptase Polymerase Chain Reaction
  • Swine
  • Swine Diseases / virology*

Substances

  • Capsid Proteins
  • DNA Primers