Dopamine coated Fe3O4 nanoparticles as enzyme mimics for the sensitive detection of bacteria

Chem Commun (Camb). 2017 Nov 14;53(91):12306-12308. doi: 10.1039/c7cc07149c.

Abstract

We report a simple and economical colorimetric bacterial sensing strategy with catalytic amplification using dopamine-capped iron oxide (Dop-Fe3O4) nanoparticles. These nanoparticles catalyse the oxidation of a chromogenic substrate in the presence of H2O2 into a green colored product. The catalytic activity of the nanoparticles is inhibited in the presence of bacteria, providing naked eye detection of bacteria at 104 cfu mL-1 and by spectrophotometric detection down to 102 cfu mL-1.

MeSH terms

  • Bacillus subtilis / isolation & purification*
  • Biosensing Techniques*
  • Catalysis
  • Colorimetry*
  • Dopamine / chemistry*
  • Escherichia coli / isolation & purification*
  • Magnetite Nanoparticles / chemistry*
  • Particle Size
  • Spectrophotometry

Substances

  • Magnetite Nanoparticles
  • Dopamine