Proton-detected solid-state NMR detects the inter-nucleotide correlations and architecture of dimeric RNA in microcrystals

Yufei Yang; ShengQi Xiang; Xiaodan Liu; Xiaojing Pei; Pengzhi Wu; Qingguo Gong; Na Li; Marc Baldus; Shenlin Wang

doi:10.1039/c7cc07483b

Proton-detected solid-state NMR detects the inter-nucleotide correlations and architecture of dimeric RNA in microcrystals

Chem Commun (Camb). 2017 Nov 30;53(96):12886-12889. doi: 10.1039/c7cc07483b.

Authors

Yufei Yang¹, ShengQi Xiang, Xiaodan Liu, Xiaojing Pei, Pengzhi Wu, Qingguo Gong, Na Li, Marc Baldus, Shenlin Wang

Affiliation

¹ College of Chemistry and Molecular Engineering, Beijing, China. [email protected].

PMID: 29099144
DOI: 10.1039/c7cc07483b

Abstract

We report a novel proton-detected MAS solid-state NMR strategy based on ¹⁵N-¹⁵N proton assisted recoupling to detect the inter-nucleotide NHN hydrogen bonds within the Watson-Crick base pairs of micro-crystallized dimeric RNA and to confirm the kissing-loop structure. This would contribute to advances in the structural determination of RNA using solid-state NMR.

MeSH terms

Crystallization
Magnetic Resonance Spectroscopy / methods*
Nucleic Acid Conformation*
Nucleotides / analysis*
Protons*
RNA / chemistry*

Substances

Nucleotides
Protons
RNA