Identification of cell cycle-targeting microRNAs through genome-wide screens

Cell Cycle. 2017;16(23):2241-2248. doi: 10.1080/15384101.2017.1380132. Epub 2017 Nov 14.

Abstract

By performing nine genome-wide microRNA (miRNA) screens, we recently uncovered a new class of miRNAs, which target multiple cyclins and cyclin-dependent kinases (CDKs). Systemic delivery of selected cell cycle-targeting miRNAs to mouse xenograft models resulted in potent anti-tumorigenic effects without affecting animals' health. Here, we provide an in-depth description of our miRNA screening methodology, analyses of selected cell cycle-targeting miRNAs, and discuss why miRNA therapy might be a viable therapeutic option for cancer patients.

Keywords: cancer; cell cycle; cyclin-dependent kinases; cyclins; microRNAs.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Adipose Tissue / metabolism
  • Animals
  • Cell Cycle
  • Cyclin-Dependent Kinases / genetics*
  • Cyclins / genetics*
  • Genome*
  • Humans
  • Mice
  • MicroRNAs / metabolism*
  • Neoplasms / genetics
  • Neoplasms / pathology

Substances

  • 3' Untranslated Regions
  • Cyclins
  • MicroRNAs
  • Cyclin-Dependent Kinases