CryoEM-based hybrid modeling approaches for structure determination

Curr Opin Microbiol. 2018 Jun:43:14-23. doi: 10.1016/j.mib.2017.10.002. Epub 2017 Nov 4.

Abstract

Recent advances in cryo-electron microscopy (cryoEM) have dramatically improved the resolutions at which vitrified biological specimens can be studied, revealing new structural and mechanistic insights over a broad range of spatial scales. Bolstered by these advances, much effort has been directed toward the development of hybrid modeling methodologies for the construction and refinement of high-fidelity atomistic models from cryoEM data. In this brief review, we will survey the key elements of cryoEM-based hybrid modeling, providing an overview of available computational tools and strategies as well as several recent applications.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Computational Biology
  • Computer Simulation
  • Cryoelectron Microscopy / methods*
  • Models, Molecular*
  • Protein Conformation