The microbial metabolite indole-3-propionic acid improves glucose metabolism in rats, but does not affect behaviour

Arch Physiol Biochem. 2018 Oct;124(4):306-312. doi: 10.1080/13813455.2017.1398262. Epub 2017 Nov 7.

Abstract

Context: We have previously shown that an antidepressant-like effect of probiotics in rats was associated with a higher plasma level of the microbial tryptophan metabolite indole-3-propionic acid (IPA).

Objective: We therefore wanted to study the isolated effect of IPA on behaviour and glucose metabolism in rats.

Methods: Male Sprague-Dawley rats were fed control or IPA-enriched diet for six weeks (n = 12 per group) and assessed in the elevated plus maze, open field and forced swim test. Blood glucose, metabolic hormones and the white blood cell (WBC) composition were analysed.

Results: IPA (mean intake 27.3 mg/kg/day) significantly lowered fasting blood glucose level by 0.42 mM (95% CI 0.11-0.73). Similarly, fasting plasma insulin levels and the homeostatic model assessment (HOMA) index of insulin resistance were reduced, whereas plasma metabolic hormones, behaviour and WBC composition remained unaffected by IPA.

Conclusions: Our findings highlight IPA as a promising candidate for treatment of metabolic disorders associated with insulin resistance.

Keywords: Blood glucose; depression; diabetes mellitus; insulin; xenobiotics.

MeSH terms

  • Animals
  • Anxiety / blood
  • Anxiety / immunology
  • Anxiety / metabolism
  • Anxiety / prevention & control
  • Behavior, Animal
  • Blood Glucose / analysis
  • Depression / blood
  • Depression / immunology
  • Depression / metabolism
  • Depression / prevention & control
  • Diabetes Mellitus, Type 2 / blood
  • Diabetes Mellitus, Type 2 / immunology
  • Diabetes Mellitus, Type 2 / metabolism
  • Diabetes Mellitus, Type 2 / prevention & control
  • Dietary Supplements* / adverse effects
  • Hyperinsulinism / blood
  • Hyperinsulinism / immunology
  • Hyperinsulinism / metabolism
  • Hyperinsulinism / prevention & control*
  • Hypoglycemic Agents / adverse effects
  • Hypoglycemic Agents / therapeutic use*
  • Indoles / adverse effects
  • Indoles / therapeutic use*
  • Insulin / blood
  • Insulin Resistance*
  • Leukocyte Count
  • Male
  • Prediabetic State / blood
  • Prediabetic State / immunology
  • Prediabetic State / metabolism
  • Prediabetic State / prevention & control*
  • Propionates / adverse effects
  • Propionates / therapeutic use*
  • Random Allocation
  • Rats, Sprague-Dawley

Substances

  • Blood Glucose
  • Hypoglycemic Agents
  • Indoles
  • Insulin
  • Propionates
  • 3-(indol-3-yl)propionic acid