Effects of a Grapevine Shoot Extract Containing Resveratrol and Resveratrol Oligomers on Intestinal Adenoma Development in Mice: In Vitro and In Vivo Studies

Mol Nutr Food Res. 2018 Jan;62(2). doi: 10.1002/mnfr.201700450. Epub 2018 Jan 9.

Abstract

Scope: Evidence suggests that the dietary consumption of plant extracts containing polyphenols might help prevent the onset of cancers of the gastrointestinal tract. In the present study, the chemopreventive and antiproliferative efficacy of a grapevine shoot extract (Vineatrol®30) containing resveratrol and resveratrol oligomers is investigated in vivo and in vitro.

Methods and results: The in vivo study is performed using ApcMin mice on a high-fat diet, which represents a model of human adenomatous polyposis, while the potential of the extract as well as some of its isolated constituents to inhibit intestinal adenoma cell proliferation in vitro is investigated using APC10.1 cells derived from an ApcMin mouse. Vineatrol®30 at a low (2.3 mg kg-1 diet) or high dose (476 mg kg-1 diet) reduces the adenoma number in male and adenoma volume in female animals. Furthermore, Vineatrol®30 as well as resveratrol and two resveratrol tetramers compromise the expansion of APC10.1 cells by reducing cell number, inducing cell cycle arrest, cellular senescence, and apoptosis. However, except for the extract, none of the isolated resveratrol oligomers is more efficacious than resveratrol in these cells.

Conclusion: Vineatrol®30 may merit further investigation as a potential dietary gastrointestinal cancer chemopreventive agent in humans.

Keywords: ApcMin mouse; chemoprevention; grapevine shoot extract; resveratrol; resveratrol oligomers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoma / metabolism
  • Adenoma / pathology
  • Adenoma / prevention & control*
  • Animals
  • Anticarcinogenic Agents / chemistry
  • Anticarcinogenic Agents / pharmacology*
  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Caspases / metabolism
  • Cell Proliferation / drug effects
  • Female
  • Intestinal Neoplasms / metabolism
  • Intestinal Neoplasms / pathology
  • Intestinal Neoplasms / prevention & control*
  • Male
  • Mice, Mutant Strains
  • Phenols / chemistry
  • Phenols / pharmacology*
  • Resveratrol / chemistry
  • Resveratrol / pharmacology*
  • Stilbenes / pharmacology

Substances

  • Anticarcinogenic Agents
  • Antineoplastic Agents, Phytogenic
  • Phenols
  • Stilbenes
  • hopeaphenol
  • vineatrol
  • Caspases
  • Resveratrol