Acute Myeloid Leukemia Immunophenotyping by Flow Cytometric Analysis

Xueyan Chen; Sindhu Cherian

doi:10.1016/j.cll.2017.07.003

Acute Myeloid Leukemia Immunophenotyping by Flow Cytometric Analysis

Clin Lab Med. 2017 Dec;37(4):753-769. doi: 10.1016/j.cll.2017.07.003. Epub 2017 Aug 31.

Authors

Xueyan Chen¹, Sindhu Cherian²

Affiliations

¹ Department of Laboratory Medicine, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195, USA. Electronic address: [email protected].
² Department of Laboratory Medicine, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195, USA.

PMID: 29128067
DOI: 10.1016/j.cll.2017.07.003

Abstract

Flow cytometry plays an indispensible role in the diagnosis and subclassification of acute myeloid leukemia (AML). Using a multiparametric approach, flow cytometry immunophenotyping has the advantage of efficiency with high sensitivity. This article reviews the general gating strategy, antibody panels for routine analysis, and additional markers for lineage assignment in the subclassification of AML. Also discussed are diagnostic immunophenotypic features of hard-to-classify entities considered within the differential diagnosis of AML. Finally, briefly presented are the principles underlying the use of flow cytometry for minimal residual disease detection.

Keywords: Acute myeloid leukemia; Flow cytometry; Minimal residual disease; Mixed phenotype acute leukemia.

Publication types

Review

MeSH terms

Flow Cytometry*
Humans
Immunophenotyping*
Leukemia, Myeloid, Acute / diagnosis*
Neoplasm, Residual