Mouse adenovirus type 1 infection of adipose tissue

Caitlyn T Molloy; Laura J Adkins; Cameron Griffin; Kanakadurga Singer; Jason B Weinberg

doi:10.1016/j.virusres.2017.11.014

Mouse adenovirus type 1 infection of adipose tissue

Virus Res. 2018 Jan 15:244:90-98. doi: 10.1016/j.virusres.2017.11.014. Epub 2017 Nov 12.

Authors

Caitlyn T Molloy¹, Laura J Adkins¹, Cameron Griffin¹, Kanakadurga Singer¹, Jason B Weinberg²

Affiliations

¹ Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI, United States.
² Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI, United States; Department of Microbiology and Immunology, University of Michigan, Ann Arbor, MI, United States. Electronic address: [email protected].

PMID: 29141203
DOI: 10.1016/j.virusres.2017.11.014

Abstract

Human adenovirus (HAdV) type 36 seropositivity has been linked to obesity in humans. That link is supported by a small number of studies using HAdV-36 infection of animals that are not natural hosts for HAdVs. In this study, we infected mice with mouse adenovirus type 1 (MAV-1), a mouse pathogen, to determine whether MAV-1 infected adipose tissue and was associated with adipose tissue inflammation and obesity. We detected MAV-1 in adipose tissue during acute MAV-1 infection, but we did not detect virus-induced increases in adipose tissue cytokine expression or histological evidence of adipose tissue inflammation during acute infection. MAV-1 did not persist in adipose tissue at later times, and we did not detect long-term adipose inflammation, increased adipose tissue mass, or body weight in infected mice. Our data indicate that MAV-1 is not associated with obesity in infected mice.

Keywords: Adenovirus; Adipose tissue; Inflammation; Mouse model; Obesity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenoviridae Infections / genetics
Adenoviridae Infections / immunology
Adenoviridae Infections / virology*
Adipose Tissue / immunology
Adipose Tissue / virology*
Animals
Body Weight
CCAAT-Enhancer-Binding Protein-beta / genetics
CCAAT-Enhancer-Binding Protein-beta / immunology
Cell Line
Chemokine CCL2 / genetics
Chemokine CCL2 / immunology
DNA, Viral / genetics*
DNA, Viral / immunology
Female
Fibroblasts / virology
Gene Expression Regulation / immunology*
Host-Pathogen Interactions*
Interferon-gamma / genetics
Interferon-gamma / immunology
Interleukin-17 / genetics
Interleukin-17 / immunology
Male
Mastadenovirus / genetics*
Mastadenovirus / growth & development
Mastadenovirus / metabolism
Mice
Mice, Inbred C57BL
PPAR gamma / genetics
PPAR gamma / immunology
Tumor Necrosis Factor-alpha / genetics
Tumor Necrosis Factor-alpha / immunology
Virus Replication

Substances

CCAAT-Enhancer-Binding Protein-beta
Ccl2 protein, mouse
Cebpb protein, mouse
Chemokine CCL2
DNA, Viral
Interleukin-17
PPAR gamma
Tumor Necrosis Factor-alpha
Interferon-gamma