Objective: To analyze the clinical significance of anti- ubiquitin C-terminal hydrolase L1(UCHL-1)autoantibodies in neuropsychiatric systemic lupus erythematosus (NPSLE). Methods: Autoantibodies in cerebrospinal fluid specimen of 56 inpatients were detected by using indirect enzyme-linked immunosorbent assay (ELISA) and the fullmedical history and clinical manifestations were analyzed retrospectively. Results: The levels of anti-UCHL-1 autoantibodies in NPSLE group were significant higher than that in other controls (P<0.05). The positive rate of anti-UCHL-1 autoantibodies in NPSLE group was 23.7% (9/38), which was higher than that in the control groups (0%). A significant difference of anti-UCHL-1 autoantibodies was observed in the patients with blood system involvement (P=0.012). The positive rates of anti-UCHL autoantibodies in the patients with negative SLE related autoantibodies including AnuA, anti-dsDNA, Acl, anti-nRNP, anti-rRNP and anti-Smantibody negative were 41.7%, 29.4%, 29.2%, 25.9%, 25.0%, 25.0%, respectively.The levels of anti-UCHL-1 autoantibodies had a positive correlation with 24-hours proteinuria (r=0.361, P=0.039). Conclusion: Anti-UCHL-1 autoantibodies had promising value in the diagnosis of neuropsychiatric systemic lupus erythematosus.
目的： 探讨抗泛素羧基末端水解酶－1(UCHL－1)抗体在神经精神性狼疮(NPSLE)诊断中的临床意义。 方法： 采用间接酶联免疫吸附试验对2014年5月至2016年3月间在郑州大学第五附属医院住院56例患者的脑脊液进行抗UCHL－1抗体检测，并分析NPSLE患者的临床资料及诊断价值。 结果： 抗UCHL－1抗体在NPSLE组中的表达水平显著高于系统性红斑狼疮(SLE)组及神经疾病对照组(P<0.05)。脑脊液中抗UCHL－1抗体阳性率在NPSLE组为23.7%(9/38)，其他对照组为0%。在抗核小体抗体、抗dsDNA抗体、抗磷脂抗体、抗nRNP抗体、抗核糖体P蛋白抗体、抗Sm抗体阴性的NPSLE患者中抗UCHL－1抗体阳性率分别为41.7%、29.4%、29.2%、25.9%、25.0%、25.0%。抗UCHL－1抗体在血液系统受累的NPSLE患者中检出率显著增高(P＝0.012)。抗UCHL－1抗体表达水平与24 h尿蛋白呈正相关(r＝0.361, P＝0.039)。 结论： 抗UCHL－1抗体对NPSLE的诊断具有一定意义。.
Keywords: Anti- ubiquitin C-terminal hydrolase L1 antibody; Indirect enzyme-linked immunosorbent assay; Neuropsychiatric systemic lupus erythematosus.