A practical molecular identification of nonfermenting Gram-negative bacteria from cystic fibrosis

Braz J Microbiol. 2018 Apr-Jun;49(2):422-428. doi: 10.1016/j.bjm.2017.07.002. Epub 2017 Nov 4.

Abstract

Identification of nonfermenting Gram-negative bacteria (NFGNB) of cystic fibrosis patients is hard and misidentification could affect clinical outcome. This study aimed to propose a scheme using polymerase chain reaction to identify NFGNB. This scheme leads to reliable identification within 3 days in an economically viable manner when compared to other methods.

Keywords: Cystic fibrosis; Diagnosis; Identification scheme; Nonfermenting Gram-negative bacteria.

MeSH terms

  • Cystic Fibrosis / complications*
  • Gram-Negative Bacteria / genetics
  • Gram-Negative Bacteria / isolation & purification*
  • Gram-Negative Bacterial Infections / diagnosis*
  • Humans
  • Molecular Diagnostic Techniques / methods*
  • Polymerase Chain Reaction / methods*
  • Time Factors