Protective effects of allicin on 1,3-DCP-induced lipid metabolism disorder in HepG2 cells

Biomed Pharmacother. 2017 Dec:96:1411-1417. doi: 10.1016/j.biopha.2017.10.125. Epub 2017 Nov 21.

Abstract

Allicin (2-propene-1-sulfinothioic acid S-2-propenyl ester), with quite a good range of hepatoprotective and antineoplastic properties, is a functional substance from garlic (Allium sativum L.) The purpose of this study was to provide evidence that allicin could protect 1,3-DCP-induced lipid metabolism disorder in HepG2 cells. Allicin reduced the accumulation of triglycerides (TG) and total cholesterol (TC) in 1,3-DCP-induced HepG2 cells. Allicin significantly increased the phosphorylation of AMP-activated protein kinase (AMPK) and down-regulated the levels of sterol regulatory element binding protein-1 (SREBP-1) and sterol regulatory element binding protein-2 (SREBP-2) in 1,3-DCP-induced HepG2 cells. Additionally, allicin had obvious recovery influence on the phosphorylation level of PKA and CREB in 1,3-DCP-induced HepG2 cells. These observations indicated that allicin alleviated lipid metabolism disorder induced by 1,3-DCP in HepG2 cells by regulating AMPK-SREBPs and PKA-CREB signaling pathways.

Keywords: 1,3-DCP; AMPK; Allicin; Lipid metabolism disorder; PKA.

MeSH terms

  • AMP-Activated Protein Kinases / metabolism
  • Cell Line, Tumor
  • Cholesterol / metabolism
  • Cyclic AMP Response Element-Binding Protein / metabolism
  • Cyclic AMP-Dependent Protein Kinases / metabolism
  • Disulfides
  • Down-Regulation / drug effects
  • Hep G2 Cells
  • Humans
  • Lipid Metabolism / drug effects*
  • Phosphorylation / drug effects
  • Protective Agents / pharmacology*
  • Signal Transduction / drug effects
  • Sterol Regulatory Element Binding Protein 1 / metabolism
  • Sulfinic Acids / pharmacology*
  • Triglycerides / metabolism

Substances

  • Cyclic AMP Response Element-Binding Protein
  • Disulfides
  • Protective Agents
  • Sterol Regulatory Element Binding Protein 1
  • Sulfinic Acids
  • Triglycerides
  • allicin
  • Cholesterol
  • Cyclic AMP-Dependent Protein Kinases
  • AMP-Activated Protein Kinases