BAD knockout provides metabolic seizure resistance in a genetic model of epilepsy with sudden unexplained death in epilepsy

Epilepsia. 2018 Jan;59(1):e1-e4. doi: 10.1111/epi.13960. Epub 2017 Nov 23.

Abstract

Metabolic alteration, either through the ketogenic diet (KD) or by genetic alteration of the BAD protein, can produce seizure protection in acute chemoconvulsant models of epilepsy. To assess the seizure-protective role of knocking out (KO) the Bad gene in a chronic epilepsy model, we used the Kcna1-/- model of epilepsy, which displays progressively increased seizure severity and recapitulates the early death seen in sudden unexplained death in epilepsy (SUDEP). Beginning on postnatal day 24 (P24), we continuously video monitored Kcna1-/- and Kcna1-/- Bad-/- double knockout mice to assess survival and seizure severity. We found that Kcna1-/- Bad-/- mice outlived Kcna1-/- mice by approximately 2 weeks. Kcna1-/- Bad-/- mice also spent significantly less time in seizure than Kcna1-/- mice on P24 and the day of death, showing that BadKO provides seizure resistance in a genetic model of chronic epilepsy.

Keywords: BAD; Kcna1 −/−; Kv1.1; metabolic seizure resistance.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Animals
  • Brugada Syndrome / etiology*
  • Brugada Syndrome / genetics*
  • Brugada Syndrome / metabolism
  • Disease Models, Animal
  • Electroencephalography
  • Epilepsy* / complications
  • Epilepsy* / genetics
  • Epilepsy* / prevention & control
  • Female
  • Kv1.1 Potassium Channel / genetics*
  • Kv1.1 Potassium Channel / metabolism
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • bcl-Associated Death Protein / deficiency*
  • bcl-Associated Death Protein / genetics

Substances

  • Bad protein, mouse
  • Kcna1 protein, mouse
  • bcl-Associated Death Protein
  • Kv1.1 Potassium Channel