Abstract
Recombinant human bone morphogenetic protein-2 with an additional s-tag domain (s-tag-BMP-2) synthesized in E. coli is characterized by higher solubility and activity than the protein without additional s-tag domain, which increases the yield during purification and simplifies protein introduction into the osteoplastic materials. The high osteoinductivity of the demineralized bone matrix with s-tag-BMP-2 was shown on the model of regeneration of cranial defects of a critical size in mice and on the model of implantation of porous titanium matrix into defects of femoral and tibial bones in rabbits.
Keywords:
critical size defect; demineralized bone matrix; regeneration of bone tissue; rhBMP-2; titanium implants.
MeSH terms
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Animals
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Biocompatible Materials / chemistry
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Biocompatible Materials / pharmacology
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Bone Morphogenetic Protein 2 / biosynthesis
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Bone Morphogenetic Protein 2 / genetics
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Bone Morphogenetic Protein 2 / pharmacology*
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Bone Regeneration / drug effects*
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Cloning, Molecular
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Femur / drug effects*
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Femur / injuries
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Gene Expression
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Implants, Experimental
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Male
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Mice
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Mice, Inbred ICR
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Rabbits
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / pharmacology*
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Skull / drug effects*
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Skull / injuries
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Tibia / drug effects*
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Tibia / injuries
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Tissue Engineering
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Tissue Scaffolds
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Titanium / chemistry
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Titanium / pharmacology
Substances
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BMP2 protein, human
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Biocompatible Materials
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Bone Morphogenetic Protein 2
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Recombinant Fusion Proteins
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Titanium