Removal of fibroblasts from human epithelial cell cultures with use of a complement fixing monoclonal antibody reactive with human fibroblasts and monocytes/macrophages

J Invest Dermatol. 1989 Feb;92(2):166-70. doi: 10.1111/1523-1747.ep12276685.

Abstract

A complement fixing IgM monoclonal antibody (1B10) that reacts with surface membrane molecules of human fibroblasts, tissue macrophages, and peripheral monocytes was produced. In Western blot analysis of detergent extracts of cultured human foreskin fibroblasts, antibody 1B10 detected protein bands of Mr 43,000 and 72-80,000. We used the 1B10 antibody with complement to eliminate most 1B10 positive nonepithelial cells from thymic epithelial (TE) cell cultures, thereby allowing us to grow highly enriched populations of human TE cells.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Monoclonal / immunology*
  • Antigens / analysis
  • Blotting, Western
  • Cell Separation / methods*
  • Cells, Cultured
  • Complement System Proteins / immunology*
  • Cytotoxicity, Immunologic
  • Epithelial Cells
  • Epithelium / immunology
  • Fibroblasts / cytology*
  • Fibroblasts / immunology
  • Humans
  • Macrophages / immunology
  • Monocytes / immunology
  • Skin / cytology
  • Thymus Gland / cytology

Substances

  • Antibodies, Monoclonal
  • Antigens
  • Complement System Proteins