Abstract
A DNA-encoded macrocyclic peptide library was designed and synthesized with 2.4 × 1012 members composed of 4-20 natural and non-natural amino acids. Affinity-based selection was performed against two therapeutic targets, VHL and RSV N protein. On the basis of selection data, some peptides were selected for resynthesis without a DNA tag, and their activity was confirmed.
MeSH terms
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Amino Acids / chemistry
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DNA / chemistry
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Drug Evaluation, Preclinical / methods
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Molecular Targeted Therapy
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Peptide Library*
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Peptides, Cyclic / chemistry*
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Peptides, Cyclic / genetics
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Peptides, Cyclic / pharmacology*
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Polymerase Chain Reaction
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Respiratory Syncytial Viruses
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Viral Proteins / antagonists & inhibitors
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Viral Proteins / chemistry
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Viral Proteins / metabolism*
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Von Hippel-Lindau Tumor Suppressor Protein / chemistry
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Von Hippel-Lindau Tumor Suppressor Protein / metabolism*
Substances
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Amino Acids
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Peptide Library
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Peptides, Cyclic
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Viral Proteins
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DNA
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Von Hippel-Lindau Tumor Suppressor Protein
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VHL protein, human