In-Depth Functional Analysis of Rodents by Full-Field Electroretinography

Methods Mol Biol. 2018:1715:207-213. doi: 10.1007/978-1-4939-7522-8_14.

Abstract

Full-field electroretinography (ERG) belongs to the gold-standard of electrophysiological test systems in ophthalmology and reflects the sum response of the entire retina to light stimulation. The assessment of the retinal function is a fundamental diagnostic technique not only in the clinical ophthalmology it is also indispensable in the ophthalmic research, in particular, in therapeutic approaches where the in vivo follow up of the benefit after treatment is absolutely necessary. Several current therapeutic approaches have demonstrated long-lasting amelioration in respective disease models and show promise for a successful translation to human patients. In this chapter we provide electroretinography protocols of experimental data which may serve as informative features for upcoming gene therapeutic approaches and clinical trials.

Keywords: Cone; Electroretinography; Gene therapy; Mouse; Rod; Rodents.

MeSH terms

  • Animals
  • Electroretinography / instrumentation
  • Electroretinography / methods*
  • Humans
  • Mice
  • Mice, Inbred C57BL
  • Retina / physiology*
  • Retinal Cone Photoreceptor Cells / metabolism
  • Retinal Rod Photoreceptor Cells / metabolism