Assessment of antioxidant activity, lipid profile, general biochemical and immune system responses of Wistar rats fed with dairy dessert containing Lactobacillus acidophilus La-5

C S Moura; P C B Lollo; P N Morato; E A Esmerino; L P Margalho; V A Santos-Junior; P T Coimbra; L P Cappato; M C Silva; A S Garcia-Gomes; D Granato; H M A Bolini; A S Sant'Ana; A G Cruz; Jaime Amaya-Farfan

doi:10.1016/j.foodres.2016.10.042

Assessment of antioxidant activity, lipid profile, general biochemical and immune system responses of Wistar rats fed with dairy dessert containing Lactobacillus acidophilus La-5

Food Res Int. 2016 Dec:90:275-280. doi: 10.1016/j.foodres.2016.10.042. Epub 2016 Oct 27.

Authors

C S Moura¹, P C B Lollo², P N Morato¹, E A Esmerino¹, L P Margalho¹, V A Santos-Junior³, P T Coimbra⁴, L P Cappato⁵, M C Silva⁴, A S Garcia-Gomes⁶, D Granato⁷, H M A Bolini¹, A S Sant'Ana¹, A G Cruz⁸, Jaime Amaya-Farfan¹

Affiliations

¹ University of Campinas (UNICAMP), Faculty of Food Engineering (FEA), 13083-862 Campinas, SP, Brazil.
² University of Campinas (UNICAMP), Faculty of Food Engineering (FEA), 13083-862 Campinas, SP, Brazil; Universidade Federal da Grande Dourados (UFGD), 79825-070 Dourados, MS, Brazil. Electronic address: [email protected].
³ Universidade Federal da Grande Dourados (UFGD), 79825-070 Dourados, MS, Brazil.
⁴ Federal Institute of Rio de Janeiro (IFRJ), Departament of Food, 20270-921 Rio de Janeiro, RJ, Brazil.
⁵ Federal Rural University of Rio de Janeiro (UFRRJ), Food Technology Department, 23890-000 Seropédica, Rio de Janeiro, Brazil.
⁶ Federal Institute of Rio de Janeiro (IFRJ), Departament of Food, 20270-921 Rio de Janeiro, RJ, Brazil; Laboratory of Integrated Studies in Protozoology, Oswaldo Cruz Institute (IOC/Fiocruz), 21040-360, Rio de Janeiro, Brazil.
⁷ State University of Ponta Grossa (UEPG), Department of Food Engineering, Av. Carlos Cavalcanti, 4748, 84030-900 Ponta Grossa, PR, Brazil.
⁸ Federal Institute of Rio de Janeiro (IFRJ), Departament of Food, 20270-921 Rio de Janeiro, RJ, Brazil. Electronic address: [email protected].

PMID: 29195882
DOI: 10.1016/j.foodres.2016.10.042

Abstract

The viability and survival of Lactobacillus acidophilus La5 under in vitro simulated gastrointestinal in probiotic dairy dessert was assessed. In addition, the effects of regular consumption of the dessert (5g/day) on the lipid profile, immune system, and antioxidant/biochemical status of Wistar rats were also evaluated after 2weeks of treatment. Adequate counts of L. acidophilus La-5 were observed regards the viability and gastrointestinal conditions. The probiotic dairy dessert was efficient in reducing the LDL-cholesterol, triacylglycerol and increased the HDL-cholesterol in serum. Aspartate amino transferase, alanine aminotransferase, total protein, albumin, heat shock proteins, immune system responses, and blood-cells counts (monocyte, lymphocyte, neutrophil and leucocyte) were not affected (p>0.05) after 15days of treatment. Overall, the probiotic dairy dessert may be a viable alternative to enhance the blood lipid profile and could be used to improve the antioxidant defenses.

Keywords: Antioxidant defense; Dairy dessert; Immune system; Probiotic bacteria.