STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods

Sci Rep. 2017 Dec 7;7(1):17189. doi: 10.1038/s41598-017-17525-5.

Abstract

The growing interest in liquid biopsies for cancer research and cell-based non-invasive prenatal testing (NIPT) invigorates the need for improved single cell analysis. In these applications, target cells are extremely rare and fragile in peripheral circulation, which makes the genetic analysis very challenging. To overcome these challenges, cell stabilization and unbiased whole genome amplification are required. This study investigates the performance of four WGA methods on single or a limited number of cells after 24 hour of Streck Cell-Free DNA BCT preservation. The suitability of the DNA, amplified with Ampli1, DOPlify, PicoPLEX and REPLI-g, was assessed for both short tandem repeat (STR) profiling and copy number variant (CNV) analysis after shallow whole genome massively parallel sequencing (MPS). Results demonstrate that Ampli1, DOPlify and PicoPLEX perform well for both applications, with some differences between the methods. Samples amplified with REPLI-g did not result in suitable STR or CNV profiles, indicating that this WGA method is not able to generate high quality DNA after Streck Cell-Free DNA BCT stabilization of the cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • B-Lymphocytes / metabolism*
  • B-Lymphocytes / pathology
  • Cell-Free Nucleic Acids / analysis*
  • DNA Copy Number Variations*
  • Female
  • Genome, Human*
  • High-Throughput Nucleotide Sequencing / methods*
  • Humans
  • Microsatellite Repeats*
  • Nucleic Acid Amplification Techniques / methods*
  • Pregnancy
  • Preservation, Biological
  • Reagent Kits, Diagnostic
  • Sequence Analysis, DNA / methods
  • Single-Cell Analysis / methods

Substances

  • Cell-Free Nucleic Acids
  • Reagent Kits, Diagnostic