Tackling Hypotheticals in Helminth Genomes

International Molecular Helminthology Annotation Network (IMHAN); IMHAN consortium authors include; Nikola Palevich; Collette Britton; Laura Kamenetzky; Makedonka Mitreva; Marina de Moraes Mourão; Sasisekhar Bennuru; Thomas Quack; Larissa Lopes Silva Scholte; Rahul Tyagi; Barton E Slatko

doi:10.1016/j.pt.2017.11.007

Tackling Hypotheticals in Helminth Genomes

Trends Parasitol. 2018 Mar;34(3):179-183. doi: 10.1016/j.pt.2017.11.007. Epub 2017 Dec 14.

Affiliations

¹ Molecular Parasitology, Animal Science, AgResearch Ltd., Grasslands Research Centre, Palmerston North, New Zealand. Electronic address: [email protected].
² Institute of Biodiversity, Animal Health and Comparative Medicine, University of Glasgow, UK.
³ Instituto de Microbiología y Parasitología Médica, Universidad de Buenos Aires Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM-UBA-CONICET), Buenos Aires, Argentina.
⁴ McDonnell Genome Institute, Washington University School of Medicine, St Louis, MO, USA; Division of Infectious Diseases, Department of Medicine, Washington University School of Medicine, St Louis, MO, USA.
⁵ Centro de Pesquisas René Rachou, FIOCRUZ, Belo Horizonte, Minas Gerais, Brazil.
⁶ NIAID, National Institutes of Health, Bethesda, MD, USA.
⁷ BFS, Institute of Parasitology, Justus Liebig University Giessen, Germany.
⁸ McDonnell Genome Institute, Washington University School of Medicine, St Louis, MO, USA.
⁹ Molecular Parasitology Division, New England Biolabs, Inc., Ipswich, MA, USA.

Abstract

Advancements in genome sequencing have led to the rapid accumulation of uncharacterized 'hypothetical proteins' in the public databases. Here we provide a community perspective and some best-practice approaches for the accurate functional annotation of uncharacterized genomic sequences.

Keywords: CRISPR; RNAi; annotation; genomes; helminth; hypothetical genes.

MeSH terms

Animals
Genome, Helminth / genetics*
Genomics / trends
Helminth Proteins / genetics
Helminths / genetics*
Molecular Sequence Annotation
Sequence Analysis, RNA

Substances

Helminth Proteins

Grants and funding

Z99 AI999999/ImNIH/Intramural NIH HHS/United States