Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation

Benjamin J Shields; Andrew Keniry; Marnie E Blewitt; Matthew P McCormack

doi:10.1007/978-1-4939-7568-6_15

Analysis of Histone Modifications in Acute Myeloid Leukaemia Using Chromatin Immunoprecipitation

Methods Mol Biol. 2018:1725:177-184. doi: 10.1007/978-1-4939-7568-6_15.

Authors

Benjamin J Shields¹, Andrew Keniry^{2

3}, Marnie E Blewitt^{2

3}, Matthew P McCormack⁴

Affiliations

¹ Australian Centre for Blood Diseases, Monash University, Melbourne, VIC, Australia.
² The Walter and Eliza Hall Institute, Parkville, VIC, Australia.
³ Department of Medical Biology, University of Melbourne, Melbourne, VIC, Australia.
⁴ Australian Centre for Blood Diseases, Monash University, Melbourne, VIC, Australia. [email protected].

PMID: 29322418
DOI: 10.1007/978-1-4939-7568-6_15

Abstract

Chromatin Immunoprecipitation (ChIP) using antibodies specific for histone modifications is a powerful technique for assessing the epigenetic states of cell populations by either quantitative PCR (ChIP-PCR) or next generation sequencing analysis (ChIP-Seq). Here we describe the procedure for ChIP of histone marks in myeloid leukaemia cell lines and the subsequent purification of genomic DNA associated with repressive and activating histone modifications for further analysis. This procedure can be widely applied to a variety of histone marks to assess both activating and repressive modifications in the context of myeloid leukaemia.

Keywords: Acute myeloid leukaemia; Chromatin; Epigenetics; Histone modifications; Immunoprecipitation.

MeSH terms

Chromatin Immunoprecipitation / methods*
High-Throughput Nucleotide Sequencing
Histones / genetics
Histones / metabolism*
Humans
Leukemia, Myeloid, Acute / genetics
Leukemia, Myeloid, Acute / metabolism*
Leukemia, Myeloid, Acute / pathology
Polymerase Chain Reaction
Protein Processing, Post-Translational*
Sequence Analysis, DNA

Substances

Histones