Impact of subchronic exposure to triclosan and/or fluoride on estrogenic activity in immature female rats: The expression pattern of calbindin-D9k and estrogen receptor α genes

Yasmina M Abd-Elhakim; Amany T Mohammed; Haytham A Ali

doi:10.1002/jbt.22027

Impact of subchronic exposure to triclosan and/or fluoride on estrogenic activity in immature female rats: The expression pattern of calbindin-D9k and estrogen receptor α genes

J Biochem Mol Toxicol. 2018 Feb;32(2). doi: 10.1002/jbt.22027. Epub 2018 Jan 11.

Authors

Yasmina M Abd-Elhakim¹, Amany T Mohammed¹, Haytham A Ali²

Affiliations

¹ Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt.
² Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig, 44511, Egypt.

PMID: 29323457
DOI: 10.1002/jbt.22027

Abstract

This study explored the influence of triclosan (TCS) in the absence and presence of sodium fluoride (NaF) on estrogenic activity and thyroid function of adolescent female rats. The results indicated that the individual exposure to TCS evoked a significant decline in T₃ and T₄ but the levels of estradiol, FSH, and LH were significantly elevated beside marked up regulation of calbindin-D9k and estrogen α mRNA expression. On the other hand, the single exposure to NaF causes insignificant changes in thyroid hormones, but evoked a trend toward an increase in both estradiol and LH levels. No significant differences in the TSH level were recorded among the experimental groups. The joint exposure to TCS and NaF induced a significant improvement in thyroid and reproductive hormone levels. Overall, these findings revealed that exposure to TCS resulted in significant endocrine and reproductive alterations in immature female rats, while TCS + NaF coexposure resulted in lessening most effects.

Keywords: calbindin-D9k; estrogen; fluoride; thyroid; triclosan.

Publication types

Comparative Study

MeSH terms

Administration, Oral
Animals
Anti-Infective Agents, Local / administration & dosage
Anti-Infective Agents, Local / toxicity
Biomarkers / blood
Biomarkers / metabolism
Endocrine Disruptors / administration & dosage
Endocrine Disruptors / toxicity*
Estrogen Receptor alpha / agonists
Estrogen Receptor alpha / genetics
Estrogen Receptor alpha / metabolism*
Estrogens / metabolism
Fallopian Tubes / drug effects
Fallopian Tubes / metabolism
Fallopian Tubes / pathology
Female
Fluorides, Topical / administration & dosage
Fluorides, Topical / toxicity*
Gene Expression Regulation, Developmental / drug effects*
Gonadotropins, Pituitary / blood
Gonadotropins, Pituitary / metabolism
Infertility, Female / chemically induced*
Infertility, Female / metabolism
Infertility, Female / pathology
Infertility, Female / physiopathology
Ovary / drug effects*
Ovary / metabolism
Ovary / pathology
Random Allocation
Rats, Sprague-Dawley
S100 Calcium Binding Protein G / genetics
S100 Calcium Binding Protein G / metabolism
Thyroid Gland / drug effects
Thyroid Gland / metabolism
Thyroid Gland / pathology
Thyroid Hormones / blood
Thyroid Hormones / metabolism
Toxicity Tests, Subchronic
Triclosan / administration & dosage
Triclosan / toxicity*
Uterus / drug effects
Uterus / metabolism
Uterus / pathology

Substances

Anti-Infective Agents, Local
Biomarkers
Endocrine Disruptors
Estrogen Receptor alpha
Estrogens
Fluorides, Topical
Gonadotropins, Pituitary
S100 Calcium Binding Protein G
S100g protein, rat
Thyroid Hormones
Triclosan