A Comparison of Real-Time Polymerase Chain Reaction Assays for the Detection of Antimicrobial Resistance Markers and Sequence Typing From Clinical Nucleic Acid Amplification Test Samples and Matched Neisseria gonorrhoeae Culture

Sex Transm Dis. 2018 Feb;45(2):92-95. doi: 10.1097/OLQ.0000000000000707.

Abstract

Real-time polymerase chain reaction (PCR) assays to detect antimicrobial resistance-associated mutations were tested on Neisseria gonorrhoeae-positive clinical samples with matched isolates. Of the nucleic acid amplification tests/cultures, 87.7% (64/73), 98.6% (72/73), and 98.4% (62/63) predicted cephalosporin, ciprofloxacin, and azithromycin susceptibilities, respectively. N. gonorrhoeae multiantigen sequence type was correctly predicted for 98.7% (79/80), and 13 of 58 N. gonorrhoeae-negative specimens showed false-positive results.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Azithromycin / pharmacology
  • Bacterial Typing Techniques
  • Cephalosporins / pharmacology
  • Ciprofloxacin / pharmacology
  • Drug Resistance, Bacterial / genetics*
  • False Positive Reactions
  • Gonorrhea / microbiology*
  • Humans
  • Microbial Sensitivity Tests
  • Multilocus Sequence Typing
  • Mutation
  • Neisseria gonorrhoeae / drug effects
  • Neisseria gonorrhoeae / genetics*
  • Real-Time Polymerase Chain Reaction
  • Sensitivity and Specificity

Substances

  • Anti-Bacterial Agents
  • Cephalosporins
  • Ciprofloxacin
  • Azithromycin