Molecular characterization and expression of complement factor I in Pelteobagrus vachellii during Aeromonas hydrophila infection

Dev Comp Immunol. 2018 May:82:66-71. doi: 10.1016/j.dci.2018.01.004. Epub 2018 Jan 9.

Abstract

Complement factor I (CFI) is a novel regulatory serine protease that plays an important role in resistance to pathogen infection. In this study, the CFI gene of Pelteobagrus vachellii (Pv-CFI) was sequenced and characterized. The full-length cDNA of 2320 bp includes a 155 bp 5'-untranslated region (UTR), a 164 bp 3'-UTR, and a 2001 bp open reading frame (ORF) encoding a 667 amino acids. Multiple sequence alignment revealed five highly conserved domains with a typical modular architecture and identical active sites in vertebrates, indicating a conserved function. Pv-CFI mRNA was constitutively expressed in all examined tissues and most abundant in liver. During infection with Aeromonas hydrophila, Pv-CFI mRNA expression was significantly up-regulated in liver at 3-24 h, spleen at 3-48 h and head kidney at 3-48 h. The results suggest Pv-CFI plays an important role in resistance to pathogenic bacteria in P. vachellii.

Keywords: Complement factor I; Immunity; Pelteobagrus vachellii; cDNA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aeromonas hydrophila / physiology*
  • Animals
  • Catfishes / genetics*
  • Catfishes / immunology
  • Cloning, Molecular
  • Complement Factor I / genetics*
  • Complement Factor I / metabolism
  • DNA, Complementary / genetics
  • Fish Proteins / genetics*
  • Fish Proteins / metabolism
  • Gram-Negative Bacterial Infections / genetics
  • Gram-Negative Bacterial Infections / immunology*
  • Immunity, Innate
  • Liver / physiology*
  • Phylogeny
  • Sequence Alignment

Substances

  • DNA, Complementary
  • Fish Proteins
  • Complement Factor I