The evaluation of drug-induced changes in left ventricular function in pentobarbital-anesthetized dogs

J Pharmacol Toxicol Methods. 2018 May-Jun:91:27-35. doi: 10.1016/j.vascn.2018.01.002. Epub 2018 Jan 9.

Abstract

Introduction: The goal of this study was to determine whether assessment of myocardial contractility and hemodynamics in an anesthetized dog model, could consistently detect drug-induced changes in the inotropic state of the heart using drugs known to have clinically relevant positive and negative effects on myocardial contractility.

Methods: Derived parameters included: diastolic, systolic and mean arterial BP, peak systolic LVP, HR, end-diastolic LVP, and LVdP/dtmax as the primary contractility index.

Results: These results demonstrate that statistically significant increases (amrinone and pimobendan) and decreases (atenolol and itraconazole) in left ventricular dP/dtmax were observed at clinically relevant exposures.

Discussion: The analysis from the current study supports the strategic use of the anesthetized dog model early in the drug Discovery process for a comprehensive cardiovascular evaluation that can include left ventricular dP/dtmax with good translation to human.

Keywords: Amrinone; Arterial blood pressure; Atenolol; Beagle dog; Heart rate; Itraconazole; Left ventricular dP/dt; Methods; Myocardial contractility; Pimobendan.

Publication types

  • Evaluation Study

MeSH terms

  • Adrenergic beta-1 Receptor Antagonists / pharmacology
  • Anesthesia / methods
  • Animals
  • Antifungal Agents / adverse effects
  • Blood Pressure / drug effects
  • Cardiotonic Agents / pharmacology
  • Depression, Chemical
  • Dogs
  • Drug Evaluation, Preclinical / methods*
  • Electrocardiography
  • Heart Ventricles / drug effects
  • Hypnotics and Sedatives / administration & dosage
  • Male
  • Models, Animal
  • Myocardial Contraction / drug effects*
  • Myocardial Contraction / physiology
  • Pentobarbital / administration & dosage
  • Ventricular Function, Left / drug effects*
  • Ventricular Function, Left / physiology

Substances

  • Adrenergic beta-1 Receptor Antagonists
  • Antifungal Agents
  • Cardiotonic Agents
  • Hypnotics and Sedatives
  • Pentobarbital