A new ultraviolet-light sensitive mutant of Neurospora crassa with unusual photoreactivation property

Mutat Res. 1985 Nov-Dec;152(2-3):161-8. doi: 10.1016/0027-5107(85)90058-2.

Abstract

A mutant, uvs-(SA3B), which shows high sensitivity to UV light segregated among the progeny in a back-cross of a presumptive MMS-sensitive mutant to a wild-type strain. At 37% survival, this mutant was approximately 5 times more sensitive to UV and also 6 times more sensitive to 4-NQO than the wild type. But it was only slightly sensitive to gamma-ray, MMS, MNNG, MTC and histidine. It showed an unusual photoreactivation response. Its time course of photorecovery was similar to the photoreactivation-defective strain upr-1 of Neurospora crassa. Mutation induction by UV at the ad-3 loci in this mutant strain was lower than that at the same loci in the wild-type strain. The uvs-(SA3B) mutant maps between met-1 and col-4 in linkage group IV, and it was not allelic with the mutagen-sensitive mutant mus-8 which is located in this area. We have concluded, therefore, that uvs-(SA3B) has resulted from mutation in a new DNA-repair gene. This new mutant was barren in homozygous crosses.

MeSH terms

  • 4-Nitroquinoline-1-oxide / pharmacology
  • Carcinogens / pharmacology*
  • Gamma Rays
  • Genetic Linkage
  • Genotype
  • Histidine / pharmacology
  • Methyl Methanesulfonate / pharmacology
  • Methylnitronitrosoguanidine / pharmacology
  • Mitomycin
  • Mitomycins / pharmacology
  • Mutagenicity Tests
  • Mutation*
  • Neurospora / radiation effects*
  • Neurospora crassa / drug effects
  • Neurospora crassa / genetics
  • Neurospora crassa / radiation effects*
  • Species Specificity
  • Ultraviolet Rays*

Substances

  • Carcinogens
  • Mitomycins
  • Methylnitronitrosoguanidine
  • Histidine
  • Mitomycin
  • 4-Nitroquinoline-1-oxide
  • Methyl Methanesulfonate