Molecular mechanisms of lncRNA SMARCC2/miR-551b-3p/TMPRSS4 axis in gastric cancer

Hao Yuan; Zhaofeng Chen; Suyang Bai; Hui Wei; Yuping Wang; Rui Ji; Qinghong Guo; Qiang Li; Yuwei Ye; Jing Wu; Yongning Zhou; Liang Qiao

doi:10.1016/j.canlet.2018.01.032

Molecular mechanisms of lncRNA SMARCC2/miR-551b-3p/TMPRSS4 axis in gastric cancer

Cancer Lett. 2018 Apr 1:418:84-96. doi: 10.1016/j.canlet.2018.01.032. Epub 2018 Jan 12.

Authors

Hao Yuan¹, Zhaofeng Chen², Suyang Bai³, Hui Wei⁴, Yuping Wang⁵, Rui Ji⁶, Qinghong Guo⁷, Qiang Li⁸, Yuwei Ye⁹, Jing Wu¹⁰, Yongning Zhou¹¹, Liang Qiao¹²

Affiliations

¹ Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, China; Key Laboratory for Gastrointestinal Diseases of Gansu Province, Lanzhou University, Lanzhou, China. Electronic address: [email protected].
² Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, China; Key Laboratory for Gastrointestinal Diseases of Gansu Province, Lanzhou University, Lanzhou, China. Electronic address: [email protected].
³ Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, China; Key Laboratory for Gastrointestinal Diseases of Gansu Province, Lanzhou University, Lanzhou, China. Electronic address: [email protected].
⁴ Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, China; Key Laboratory for Gastrointestinal Diseases of Gansu Province, Lanzhou University, Lanzhou, China. Electronic address: [email protected].
⁵ Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, China; Key Laboratory for Gastrointestinal Diseases of Gansu Province, Lanzhou University, Lanzhou, China. Electronic address: [email protected].
⁶ Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, China; Key Laboratory for Gastrointestinal Diseases of Gansu Province, Lanzhou University, Lanzhou, China. Electronic address: [email protected].
⁷ Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, China; Key Laboratory for Gastrointestinal Diseases of Gansu Province, Lanzhou University, Lanzhou, China. Electronic address: [email protected].
⁸ Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, China; Key Laboratory for Gastrointestinal Diseases of Gansu Province, Lanzhou University, Lanzhou, China. Electronic address: [email protected].
⁹ Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, China; Key Laboratory for Gastrointestinal Diseases of Gansu Province, Lanzhou University, Lanzhou, China. Electronic address: [email protected].
¹⁰ Department of Gastroenterology, Beijing Shijitan Hospital, Capital Medical University, Beijing, China. Electronic address: [email protected].
¹¹ Department of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou, China; Key Laboratory for Gastrointestinal Diseases of Gansu Province, Lanzhou University, Lanzhou, China. Electronic address: [email protected].
¹² Storr Liver Centre, Westmead Institute for Medical Research, University of Sydney and Westmead Hospital, Westmead, NSW 2145, Australia. Electronic address: [email protected].

PMID: 29337109
DOI: 10.1016/j.canlet.2018.01.032

Abstract

Decreased expression of miR-551b-3p has been identified in gastric cancer tissues but its biological role and underlying mechanism in this malignancy is poorly understood. In this study, we show that the expression of miR-551b-3p negatively correlates with the depth of tumour invasion and lymphatic metastasis, but it positively correlates with tumour differentiation and the patient survival. MiR-551b-3p negatively affects the proliferation, mobility and invasiveness of gastric cancer cells. LncRNA SMARCC2 inhibits the expression of miR-551b-3p through binding to its mRNA response elements in gastric cancer cells. Overexpression of LncRNA SMARCC2 enhances the proliferation and migration of gastric cancer cells, while inhibition of LncRNA SMARCC2 does the opposite. TMPRSS4 is a direct target gene of miR-551b-3p. We conclude that miR-551b-3p functions as a tumour suppressor gene in gastric cancer, and its function is regulated by LncRNA SMARCC2/miR-551b-3p/TMPRSS4 axis.

Keywords: Gastric cancer; TMPRSS4; lncRNA SMARCC2; miR-551b-3p.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Cell Line, Tumor
Cell Proliferation / genetics
Female
Gene Expression Regulation, Neoplastic
Genes, Tumor Suppressor
HEK293 Cells
Humans
Male
Membrane Proteins / genetics*
Membrane Proteins / metabolism
Mice, Inbred BALB C
Mice, Nude
MicroRNAs / genetics*
Middle Aged
RNA, Long Noncoding / genetics*
Serine Endopeptidases / genetics*
Serine Endopeptidases / metabolism
Stomach Neoplasms / genetics*
Stomach Neoplasms / metabolism
Stomach Neoplasms / pathology
Survival Analysis
Transplantation, Heterologous

Substances

MIRN-551 microRNA, human
Membrane Proteins
MicroRNAs
RNA, Long Noncoding
Serine Endopeptidases
TMPRSS4 protein, human