CD41 immune staining of micromegakaryocytes improves the diagnosis of myelodysplastic syndrome and differentiation from pancytopenia

Leuk Res. 2018 Mar:66:15-19. doi: 10.1016/j.leukres.2017.10.004. Epub 2017 Oct 18.

Abstract

A morphometric analysis was performed on aspirated clots of bone marrow to identify the presence of micromegakaryocytes after immune staining with a monoclonal antibody raised against CD41. Quantitative and qualitative abnormalities of micromegakaryocytes were assessed based on both standard staining and CD41 immune staining. Micromegakaryocytes were largely detected in bone marrow from patients with myelodysplastic syndrome (MDS), while almost no micromegakaryocytes were present in aplastic anemia. CD41 immune staining clearly improved the efficiency of micromegakaryocyte detection under any conditions, showing strong potential as a tool for the auxiliary diagnosis of MDS and differentiation of MDS from pancytopenia, particularly aplastic anemia(AA).

Keywords: Aplastic anemia; CD41 immune staining; Micromegakaryocytes; Myelodysplastic syndrome; Wright’s staining.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Bone Marrow Cells* / metabolism
  • Bone Marrow Cells* / pathology
  • Diagnosis, Differential
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Megakaryocytes* / metabolism
  • Megakaryocytes* / pathology
  • Middle Aged
  • Myelodysplastic Syndromes* / diagnosis
  • Myelodysplastic Syndromes* / metabolism
  • Myelodysplastic Syndromes* / pathology
  • Pancytopenia / diagnosis
  • Pancytopenia / metabolism
  • Pancytopenia / pathology
  • Platelet Membrane Glycoprotein IIb / metabolism*

Substances

  • Platelet Membrane Glycoprotein IIb