Using Chlorella vulgaris to treat toxic excess sludge extract, and identification of its response mechanism by proteomics approach

Lu Wang; Hualin Wang; Xiurong Chen; Yan Xu; Tianjun Zhou; Xiaoxiao Wang; Qian Lu; Roger Ruan

doi:10.1016/j.biortech.2018.01.039

Using Chlorella vulgaris to treat toxic excess sludge extract, and identification of its response mechanism by proteomics approach

Bioresour Technol. 2018 Apr:253:188-196. doi: 10.1016/j.biortech.2018.01.039. Epub 2018 Jan 9.

Authors

Lu Wang¹, Hualin Wang², Xiurong Chen², Yan Xu², Tianjun Zhou², Xiaoxiao Wang², Qian Lu³, Roger Ruan⁴

Affiliations

¹ State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai 200237, PR China; National Engineering Laboratory for High-concentration Refractory Organic Wastewater Treatment Technologies (NELHROWTT), East China University of Science and Technology, Shanghai 200237, PR China; Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Avenue, St. Paul, MN 55108, United States.
² State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process, East China University of Science and Technology, Shanghai 200237, PR China; National Engineering Laboratory for High-concentration Refractory Organic Wastewater Treatment Technologies (NELHROWTT), East China University of Science and Technology, Shanghai 200237, PR China.
³ Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Avenue, St. Paul, MN 55108, United States.
⁴ Center for Biorefining and Department of Bioproducts and Biosystems Engineering, University of Minnesota, 1390 Eckles Avenue, St. Paul, MN 55108, United States. Electronic address: [email protected].

PMID: 29353749
DOI: 10.1016/j.biortech.2018.01.039

Abstract

Chlorella vulgaris was cultivated in varying proportions of toxic sludge extracts obtained from a sequencing batch reactor for treating synthetic wastewater containing chlorophenols. C. vulgaris could reduce the ecotoxicity from sludge extracts, and a positive correlation was noted between ecotoxicity removal and total organic carbon removal. In terms of cell density, the optimal proportion of sludge extracts required for the cultivation of C. vulgaris was lower than 50%. The correlation between protein content in per 10⁶ algae and inhibition extent of ecotoxicity of the 5 groups on the day of inoculation (0.9182, p < .05) indicated a positive relationship between algal protein secretion and ecotoxicity. According to the protein expression and differential protein expression analysis, we concluded that C. vulgaris produced proteins that involved in the stress response/redox system and energy metabolism/biosynthesis to respond to the toxic environment and some other proteins related to mixotrophic metabolism.

Keywords: Chlorella vulgaris; Excess sludge; Proteomics analysis; Response mechanism; Toxicity removal.

MeSH terms

Chlorella
Chlorella vulgaris*
Chlorophenols
Proteomics*
Sewage*
Wastewater

Substances

Chlorophenols
Sewage
Waste Water