IL-1R2 deficiency suppresses dextran sodium sulfate-induced colitis in mice via regulation of microbiota

Kaori Yoshida; Masanori A Murayama; Kenji Shimizu; Ce Tang; Nanako Katagiri; Kenzo Matsuo; Fumio Fukai; Yoichiro Iwakura

doi:10.1016/j.bbrc.2018.01.116

IL-1R2 deficiency suppresses dextran sodium sulfate-induced colitis in mice via regulation of microbiota

Biochem Biophys Res Commun. 2018 Feb 12;496(3):934-940. doi: 10.1016/j.bbrc.2018.01.116. Epub 2018 Feb 2.

Authors

Kaori Yoshida¹, Masanori A Murayama², Kenji Shimizu³, Ce Tang², Nanako Katagiri², Kenzo Matsuo⁴, Fumio Fukai⁵, Yoichiro Iwakura⁶

Affiliations

¹ Center for Animal Disease Models, Research Institute for Biomedical Sciences (RIBS), Tokyo University of Science (TUS), Chiba, 278-0022, Japan; Department of Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, TUS, Chiba, 278-0022, Japan.
² Center for Animal Disease Models, Research Institute for Biomedical Sciences (RIBS), Tokyo University of Science (TUS), Chiba, 278-0022, Japan.
³ Center for Animal Disease Models, Research Institute for Biomedical Sciences (RIBS), Tokyo University of Science (TUS), Chiba, 278-0022, Japan; Division of Immune Regulation, Institute for Genome Research, Tokushima University, Tokushima, 770-8503, Japan.
⁴ Center for Animal Disease Models, Research Institute for Biomedical Sciences (RIBS), Tokyo University of Science (TUS), Chiba, 278-0022, Japan; Division of Molecular Immunology, Medical Mycology Research Center, Chiba University, Chiba, 260-8673, Japan.
⁵ Department of Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, TUS, Chiba, 278-0022, Japan.
⁶ Center for Animal Disease Models, Research Institute for Biomedical Sciences (RIBS), Tokyo University of Science (TUS), Chiba, 278-0022, Japan. Electronic address: [email protected].

PMID: 29366788
DOI: 10.1016/j.bbrc.2018.01.116

Abstract

Ulcerative colitis (UC) is an inflammatory disease of the colon. IL1R2, which encodes IL-1 receptor type 2 (IL-1R2), was reported as a risk gene for UC. To elucidate the roles of IL-1R2 in the development of colitis, we examined the development of dextran sodium sulfate-induced colitis, a mouse model for UC using Il1r2^-/- mice. We found the severity score of colitis was milder in Il1r2^-/- mice compared with wild-type (WT) mice when they were housed separately, however the severity score was similar when they were housed in a cage. In the separate housing condition, relative contents of Actinobacteria and Bacilli in feces of Il1r2^-/- mice were lower than that of WT mice. Furthermore, IL-1β induced the expression of antimicrobial peptides (AMPs) from colon. Thus, we show that IL-1R2 is harmful for the development of colitis, because IL-1R2 promotes the growth of proinflammatory intestinal microbiota by suppressing IL-1β-induced AMP production.

Keywords: Colitis; IL-1R2; Microbiota.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antimicrobial Cationic Peptides / immunology*
Colitis / chemically induced
Colitis / immunology*
Colitis / pathology
Dextran Sulfate
Female
Gastrointestinal Microbiome / immunology*
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Receptors, Interleukin-1 / genetics
Receptors, Interleukin-1 / immunology*

Substances

Antimicrobial Cationic Peptides
Receptors, Interleukin-1
interleukin-36 receptor, mouse
Dextran Sulfate