Abstract
Dendritic cells (DCs) produce major histocompatibility complex II (MHCII) in large amounts to function as professional antigen presenting cells. Paradoxically, DCs also ubiquitinate and degrade MHCII in a constitutive manner. Mice deficient in the MHCII-ubiquitinating enzyme membrane-anchored RING-CH1, or the ubiquitin-acceptor lysine of MHCII, exhibit a substantial reduction in the number of regulatory T (Treg) cells, but the underlying mechanism was unclear. Here we report that ubiquitin-dependent MHCII turnover is critical to maintain homeostasis of lipid rafts and the tetraspanin web in DCs. Lack of MHCII ubiquitination results in the accumulation of excessive quantities of MHCII in the plasma membrane, and the resulting disruption to lipid rafts and the tetraspanin web leads to significant impairment in the ability of DCs to engage and activate thymocytes for Treg cell differentiation. Thus, ubiquitin-dependent MHCII turnover represents a novel quality-control mechanism by which DCs maintain homeostasis of membrane domains that support DC's Treg cell-selecting function.
© 2018 Oh et al.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adoptive Transfer
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Animals
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Cell Communication*
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Cell Differentiation
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Cells, Cultured
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Coculture Techniques
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Dendritic Cells / enzymology*
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Dendritic Cells / immunology
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Dendritic Cells / pathology
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Histocompatibility Antigens Class II / genetics
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Histocompatibility Antigens Class II / immunology
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Histocompatibility Antigens Class II / metabolism*
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Homeostasis
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Lymphocyte Activation
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Membrane Microdomains / enzymology*
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Membrane Microdomains / immunology
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Membrane Microdomains / pathology
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Mice, Inbred BALB C
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Mice, Inbred C57BL
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Mice, Knockout
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Phenotype
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Receptors, Antigen, T-Cell / genetics
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Receptors, Antigen, T-Cell / immunology
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Receptors, Antigen, T-Cell / metabolism
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Signal Transduction
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T-Lymphocytes, Regulatory / immunology
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T-Lymphocytes, Regulatory / metabolism*
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T-Lymphocytes, Regulatory / transplantation
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Tetraspanins / immunology
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Tetraspanins / metabolism*
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Thymocytes / immunology
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Thymocytes / metabolism*
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Ubiquitin-Protein Ligases / genetics
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Ubiquitin-Protein Ligases / immunology
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Ubiquitin-Protein Ligases / metabolism*
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Ubiquitination
Substances
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Histocompatibility Antigens Class II
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Receptors, Antigen, T-Cell
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Tetraspanins
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MARCH1 protein, mouse
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Ubiquitin-Protein Ligases