Rewiring T-cell responses to soluble factors with chimeric antigen receptors

Nat Chem Biol. 2018 Mar;14(3):317-324. doi: 10.1038/nchembio.2565. Epub 2018 Jan 29.

Abstract

Chimeric antigen receptor (CAR)-expressing T cells targeting surface-bound tumor antigens have yielded promising clinical outcomes, with two CD19 CAR-T cell therapies recently receiving FDA approval for the treatment of B-cell malignancies. The adoption of CARs for the recognition of soluble ligands, a distinct class of biomarkers in physiology and disease, could considerably broaden the utility of CARs in disease treatment. In this study, we demonstrate that CAR-T cells can be engineered to respond robustly to diverse soluble ligands, including the CD19 ectodomain, GFP variants, and transforming growth factor beta (TGF-β). We additionally show that CAR signaling in response to soluble ligands relies on ligand-mediated CAR dimerization and that CAR responsiveness to soluble ligands can be fine-tuned by adjusting the mechanical coupling between the CAR's ligand-binding and signaling domains. Our results support a role for mechanotransduction in CAR signaling and demonstrate an approach for systematically engineering immune-cell responses to soluble, extracellular ligands.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD19 / metabolism
  • Biomarkers / metabolism
  • CD4-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / cytology
  • Cytokines / metabolism
  • Green Fluorescent Proteins / metabolism
  • Humans
  • Immunosuppressive Agents / pharmacology
  • Ligands
  • Lymphoma, B-Cell / drug therapy
  • Protein Domains
  • Protein Engineering
  • Protein Multimerization
  • Receptors, Chimeric Antigen / metabolism*
  • T-Lymphocytes / cytology*
  • Transforming Growth Factor beta / metabolism

Substances

  • Antigens, CD19
  • Biomarkers
  • Cytokines
  • Immunosuppressive Agents
  • Ligands
  • Receptors, Chimeric Antigen
  • Transforming Growth Factor beta
  • Green Fluorescent Proteins