Abstract
Laboratory evolution of alcohol dehydrogenase produced enzyme variants with improved turnover numbers with a vicinal 1,2-diol and its corresponding hydroxyketone. Crystal structure and transient kinetics analysis aids in rationalizing the new functions of these variants.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Alcohol Dehydrogenase / chemistry
-
Alcohol Dehydrogenase / genetics*
-
Alcohol Dehydrogenase / metabolism*
-
Catalytic Domain
-
Directed Molecular Evolution* / methods
-
Ethylene Glycols / chemistry
-
Ethylene Glycols / metabolism*
-
Fatty Alcohols / chemistry
-
Fatty Alcohols / metabolism*
-
Mutagenesis
-
Oxidation-Reduction
-
Protein Conformation
-
Rhodococcus / chemistry
-
Rhodococcus / enzymology*
-
Rhodococcus / genetics
-
Rhodococcus / metabolism
-
Stereoisomerism
-
Substrate Specificity
Substances
-
Ethylene Glycols
-
Fatty Alcohols
-
styrene glycol
-
acyloin
-
Alcohol Dehydrogenase