Extracellular RNA Isolation from Cell Culture Supernatant

Sawen Bakr; Bridget Simonson; Kirsty M Danielson; Saumya Das

doi:10.1007/978-1-4939-7652-2_3

Extracellular RNA Isolation from Cell Culture Supernatant

Methods Mol Biol. 2018:1740:23-34. doi: 10.1007/978-1-4939-7652-2_3.

Authors

Sawen Bakr¹, Bridget Simonson¹, Kirsty M Danielson^{2

1}, Saumya Das³

Affiliations

¹ Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA, USA.
² University of Otago Wellington, Wellington, New Zealand.
³ Cardiovascular Research Center, Massachusetts General Hospital, Boston, MA, USA. [email protected].

PMID: 29388133
DOI: 10.1007/978-1-4939-7652-2_3

Abstract

Extracellular RNAs are emerging as novel biomarkers and mediators of intercellular communication. Various methods to isolate RNA from biofluids and cell culture supernatants have been previously used by investigators. Here, we describe several standardized protocols for the isolation of RNAs from cell culture supernatants that utilize commercially available kits and reagents.

Keywords: Cell culture supernatant; Extracellular RNA; Extracellular vesicles; Plasma; RNA isolation.

MeSH terms

Animals
Cell Culture Techniques*
Chemical Precipitation
Extracellular Vesicles / metabolism*
Filtration / methods
Humans
Molecular Biology / methods
RNA / analysis
RNA / isolation & purification*
Ultracentrifugation / methods

Substances

RNA