Whole Genome DNA Methylation Analysis of Human Glioblastoma Using Illumina BeadArrays

Jonathan Serrano; Matija Snuderl

doi:10.1007/978-1-4939-7659-1_2

Whole Genome DNA Methylation Analysis of Human Glioblastoma Using Illumina BeadArrays

Methods Mol Biol. 2018:1741:31-51. doi: 10.1007/978-1-4939-7659-1_2.

Authors

Jonathan Serrano¹, Matija Snuderl²

Affiliations

¹ Department of Pathology, New York University School of Medicine, New York, NY, USA.
² Department of Pathology, New York University School of Medicine, New York, NY, USA. [email protected].

PMID: 29392688
DOI: 10.1007/978-1-4939-7659-1_2

Abstract

In this chapter, we describe the use of Illumina^® Infinium^® HD Assay in conjunction with Illumina's EPIC Methylation 8-sample array platform to obtain glioblastoma molecular profiles. The procedure spans four days, and can be performed by a single laboratory technician. Starting with as little as 250 ng of DNA input, this method allows the flexibility to begin with DNA derived from either formalin-fixed, paraffin-embedded (FFPE) or fresh tissue and is compatible with an Illumina iScan or HiScan system.

Keywords: Array; DNA; DNA methylation; FFPE; GBM; Illumina; Multiplex; Profiling; Subgroup classification; Tumor.

MeSH terms

DNA Methylation*
Gene Dosage
Genome, Human*
Genomics* / methods
Glioblastoma / genetics*
High-Throughput Nucleotide Sequencing
Humans
Nucleic Acid Amplification Techniques
Oligonucleotide Array Sequence Analysis