Abstract
Here we report an example of a protein-PEG conjugate with a biotin tag cleavable by lipase-catalyzed hydrolysis. Very mild cleavage conditions, heterogeneous, easily separable catalysts, and traceless design make this method attractive for the preparation and purification of PEGylated proteins.
MeSH terms
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Biocatalysis
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Biotin / chemistry
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Biotin / metabolism*
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Hydrolysis
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Lactoglobulins / chemistry
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Lactoglobulins / metabolism*
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Lipase / chemistry
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Lipase / metabolism*
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Molecular Structure
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Polyethylene Glycols / chemistry
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Polyethylene Glycols / metabolism*
Substances
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Lactoglobulins
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Polyethylene Glycols
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Biotin
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Lipase