Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography

Xin-Ying Li; Mei Yang; Jie-Yun Huang; Xiao-Xue Yu; Min-Qian Zhao; Zhi-Kun Liang; Zhi-Sheng Xie; Xin-Jun Xu

doi:10.1016/j.jpha.2013.07.002

Preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus by high-speed counter-current chromatography

J Pharm Anal. 2013 Dec;3(6):429-433. doi: 10.1016/j.jpha.2013.07.002. Epub 2013 Jul 24.

Authors

Xin-Ying Li^{1

2}, Mei Yang^{1

2}, Jie-Yun Huang^{1

2}, Xiao-Xue Yu^{1

2}, Min-Qian Zhao^{1

2}, Zhi-Kun Liang^{1

2}, Zhi-Sheng Xie^{1

2}, Xin-Jun Xu^{1

2}

Affiliations

¹ School of Pharmaceutical Sciences, Sun Yat-Sen University, No. 132, East Waihuan Rd., Guangzhou Higher Education Mega Center, Guangzhou 510006, China.
² Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China.

Abstract

A high-speed counter-current chromatography (HSCCC) method was successfully developed for the preparative separation and purification of deoxyschizandrin from Schisandrae Sphenantherae Fructus in one step. The purity of deoxyschizandrin was 98.5%, and the structure was identified by MS, UV and NMR. This method was simple, fast, convenient and appropriate to prepare pure compound as reference substances for related research on Schisandrae Sphenantherae Fructus.

Keywords: Deoxyschizandrin; High-speed counter-current chromatography (HSCCC); Schisandrae Sphenantherae Fructus.