Abstract
The present study aimed to detect the major virulence determinants of Helicobacter pylori, the gastric bacteria by polymerase chain reaction from genomic DNA of 314 gastric biopsies from dyspeptic patients in 2015-2016 through upper gastrointestinal endoscopy. In 153 cases out of 314, the high prevalence of oipA gene followed by cagA-vacA s1 m1 combined genotypes was found in mostly gastritis/duodenitis patients followed by the peptic ulcer and normal patients. Therefore, the clinical significance of the virulence markers of H. pylori associated with the severe forms of gastroduodenal diseases is still a matter of controversy since the endoscopically normal patients were found to harbour the virulent genes.
MeSH terms
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Adult
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Antigens, Bacterial / genetics
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Bacterial Outer Membrane Proteins / genetics
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Bacterial Proteins / genetics
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Biopsy
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Dyspepsia / diagnosis
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Dyspepsia / microbiology
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Endoscopy, Gastrointestinal
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Female
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Gastric Mucosa / microbiology*
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Helicobacter Infections / diagnosis*
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Helicobacter Infections / microbiology
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Helicobacter pylori / genetics*
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Helicobacter pylori / isolation & purification*
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Hospitals
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Humans
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India
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Male
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Middle Aged
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Molecular Diagnostic Techniques / methods*
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Pathology, Molecular / methods
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Polymerase Chain Reaction / methods
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Virulence Factors / analysis*
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Virulence Factors / genetics
Substances
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Antigens, Bacterial
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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OipA protein, Helicobacter pylori
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VacA protein, Helicobacter pylori
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Virulence Factors
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cagA protein, Helicobacter pylori