Diagnostic performances of the Xpert MTB/RIF in Brazil

Respir Med. 2018 Jan:134:12-15. doi: 10.1016/j.rmed.2017.11.012. Epub 2017 Nov 21.

Abstract

Background and objectives: As for all tests, the diagnostic performances of Xpert MTB/RIF might be different in settings with different tuberculosis prevalence. Aim of the study is to evaluate the performances of Xpert MTB/RIF to diagnose tuberculosis in Brazil, where 407 culture-confirmed tuberculosis patients were retrospectively enrolled in Rio Grande do Sul, between 2015 and 2016.

Methods: Sensitivity, specificity, positive and negative predictive values of the test were calculated and a logistic regression analysis was performed to assess the role played by explanatory variables in the occurrence of true positive and negative diagnostic results.

Results: Sensitivity of Xpert MTB/RIF was 100.0%, specificity 92.8%; positive and negative predictive values were 71.4% and 100.0%, respectively. In the HIV- infected sub-group specificity was 59.3%. In the multivariate logistic regression analysis, true positivity was associated with increasing age (1.0; p-value: 0.02) while true positivity and negativity were negatively associated with alcohol abuse.

Conclusions: Xpert is sensitive and specific in the Brasilian settings.

Keywords: Brazil; Diagnosis; HIV; Tuberculosis; Xpert MTB/RIF.

Publication types

  • Evaluation Study

MeSH terms

  • Adult
  • Antibiotics, Antitubercular / pharmacology
  • Brazil
  • Coinfection
  • Drug Resistance, Bacterial
  • Female
  • HIV Infections / complications
  • Humans
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Mycobacterium tuberculosis / drug effects
  • Mycobacterium tuberculosis / isolation & purification
  • Opportunistic Infections / complications
  • Opportunistic Infections / diagnosis
  • Predictive Value of Tests
  • Retrospective Studies
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Rifampin / pharmacology
  • Sensitivity and Specificity
  • Tuberculosis / complications
  • Tuberculosis / diagnosis*

Substances

  • Antibiotics, Antitubercular
  • Rifampin